Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Leukocyte Selectin (also known as L-Selectin) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human L-Selectin antibody. L-Selectin present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human L-Selectin Antibody is added and binds to L-Selectin in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated L-Selectin antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human L-Selectin. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm